Linearity of Calibration Curve in Gel Permeation Chromatography and Mixed Gel Column
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چکیده
منابع مشابه
High Performance Gel Permeation Chromatography of Polymers
The determination of the molecular weight distribution by gel permeation chromatography requires the establishment of a molecular weight calibration and efficient columns in order to minimise chromatogram broadening. The separation process is described in terms of steric exclu— sion, retardation and partial exclusion mechanisms in order to show how the universal calibration method may be influe...
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O I 'H, I H H I in M I i I M' PROI'I H I Y ()( Hf R I'.Ul 1 AHN If rVVJf ST t'S GOVF.RNM NT, .vi.l i<; irMwi! for tlw niUx itvit ion ni sut li (.arsons nnl> ns ficcj to I nnw irs ( -inlrms m Mie crnifSP of Tln-ir d^i' n' .lulirs. '^ny [>erson finrlitij; this ilocurnpnl ■.houlil li.vm ii lu .i British force? unit .if to .1 (»ilirp smtirti hi its safe rpnirn 10 the MINISTRY l)[ ÜI f ( Nil , HQ Se...
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A major problem in gel permeation chromatography (GPC) or size exclusion chromatography is non-specific binding of applied proteins to the column matrix (stationary phase). We have tested an aqueous arginine solution as the GPC mobile phase on silica-based and polymer-based columns, using mouse monoclonal antibody and recombinant human activin, interleukin-6, basic fibroblast growth factor, and...
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Rat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivity peaks. Labeled peptides, tyrosine, and iodide were also resolved by this method. Isolated lipop...
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ژورنال
عنوان ژورنال: Kobunshi Kagaku
سال: 1970
ISSN: 1884-8079,0023-2556
DOI: 10.1295/koron1944.27.33